[qwiz style=”width: auto !important; min-height: auto !important; border-width: 4px !important; border-color: #0099cc !important; ” align=”center”]

[h] Biochemistry Flashcards

[i] Master this session in just 5 minutes.

[q] ………. in Eukaryotes makes rRNA (most numerous RNA, rampant).

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFJOQSBwb2x5bWVyYXNlIEku[Qq]

[q] ………. in Eukaryotes makes mRNA (largest RNA, massive).

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFJOQSBwb2x5bWVyYXNlIElJLg==[Qq]

[q] ………. in Eukaryotes makes 5S rRNA, tRNA (smallest RNA, tiny).

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFJOQSBwb2x5bWVyYXNlIElJSS4=[Qq]

[q] α-amanitin, found in Amanita phalloides (death cap mushrooms), inhibits ……….. (halting mRNA synthesis). Causes severe hepatotoxicity if ingested.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFJOQSBwb2x5bWVyYXNlIElJLg==[Qq]

[q] ………. inhibits RNA polymerase in both prokaryotes and eukaryotes.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IEFjdGlub215Y2luIEQu[Qq]

[q] ……….. inhibits DNA-dependent RNA polymerase in prokaryotes.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFJpZmFtcGluLg==[Qq]

[q] Though ………. are not directly translated into protein, its mutations can cause abnormal gene expression by altering the ability of RNA polymerase and transcription factors to bind.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IHByb21vdGVycy4=[Qq]

[q] In ………., most genes are composed of coding segments (exons) interrupted by noncoding segments (introns). Both exons and introns are transcribed in the nucleus. Introns are removed during processing of the RNA molecule in the nucleus.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IGV1a2FyeW90ZXMu[Qq]

[q] A 7-methylguanosine cap is added to the 5′ end while the RNA molecule is still being synthesized. The cap structure function as ………….?

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IGEgcmlib3NvbWUtYmluZGluZyBzaXRlIGFuZCBhbHNvIGhlbHBzIHRvIHByb3RlY3QgdGhlIG1STkEgY2hhaW4gZnJvbSBkZWdyYWRhdGlvbi4=[Qq]

[q] A poly-A tail is attached to the 3′ end. In this process, poly-A polymerase adds the poly-A tail (about 200 As) to the new 3′ end. The poly-A tail function is ………….?

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IHByb3RlY3RzIHRoZSBtZXNzYWdlIGFnYWluc3QgcmFwaWQgZGVncmFkYXRpb24gYW5kIGFpZHMgaW4gaXRzIHRyYW5zcG9ydCB0byB0aGUgY3l0b3BsYXNtLg==[Qq]

[q] Antibodies to spliceosomal snRNPs (anti-Smith antibodies) are highly specific for ……..?

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFNMRS4=[Qq]

[q] Synthetic …….. sequences can be introduced into cells to silence specific pathogenic genes (c-Myc oncogene) and are being explored as possible therapeutic agents.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IHNpUk5BLg==[Qq]

[q] ………. is where RNA polymerase II and multiple other transcription factors bind to DNA upstream from gene locus (AT-rich upstream sequence with TATA and CAAT boxes). Its mutation commonly results in dramatic ↓ in level of gene transcription.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IFByb21vdGVyIFNpdGUu[Qq]

[q] ……… may be located close to, far from, or even within (in an intron) the gene whose expression it regulates (variable locations).

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IEVuaGFuY2VycyBhbmQgc2lsZW5jZXJzLg==[Qq]

[q] The lac operon is negatively regulated by binding of the ……… protein to the operator locus.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IHJlcHJlc3Nvci4=

[q] The lac operon is Positively regulated by …….. binding upstream from the promoter region.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IGNBTVAtQ0FQLg==

[q] ……….. —-> unbinds repressor protein from repressor/operator site —> ↑ transcription.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IEhpZ2ggbGFjdG9zZS4=

[q] ……… —-> ↑ adenylate cyclase activity —> ↑ generation of cAMP from ATP —-> activation of catabolite activator protein (CAP) —-> ↑ transcription.

[c]IFNob3cgbWUgdG hlIGFuc3dlcg==[Qq]

[f]IExvdyBnbHVjb3NlLg==[Qq]

[x][restart]

[/qwiz]