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[h] Biochemistry Flashcards

[i] Master this session in just 5 minutes.

[q] ……….. recognize double-stranded DNA sequences called palindromes (inverted repeats) usually of four to eight base pairs in length.

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[q] …….. can be identified by examining the sequence of only one strand. Draw a line through the center of the sequence (through the central base for palindromes with an odd number of nucleotides). If the sequence is folded along this line, the bases should pair.

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[q] ……….. is used for detection and visualization of specific DNA fragment using radiolabeled DNA probe.

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[q] ………. is similar to Southern blot, except that an RNA sample is electrophoresed. Useful for studying mRNA levels, which are reflective of gene expression.

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[q] ………. is used for detection and visualization of specific protein using Labeled antibody.

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[q] ……….. identifies DNA-binding proteins (transcription factors like c-Juna nd c-Fos, Histones) using labeled oligonucleotide probes.

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[q] ……… is a molecular biology lab procedure used to amplify a desired fragment of DNA. Also Useful as a diagnostic tool (neonatal HIV, herpes encephalitis).

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[q] Polymerase chain reaction requires …….. that are complementary to the regions of DNA flanking the segment of interest.

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[q] ………. is a Laboratory technique to assess size, granularity, and protein expression (immunophenotype) of individual cells in a sample. Commonly used in workup of hematologic abnormalities (paroxysmal nocturnal hemoglobinuria, fetal RBCs in mother’s blood) and immunodeficiencies (CD4 cell count in HIV).

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[q] ……… is a genome editing tool derived from bacteria. Not used clinically. Potential applications include removing virulence factors from pathogens, replacing disease-causing alleles of genes with healthy variants, and specifically targeting tumor cells.

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[q] …….. is used to profile gene expression levels of thousands of genes simultaneously to study certain diseases and treatments. Able to detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) for a variety of applications including genotyping, clinical genetic testing, forensic analysis, cancer mutations, and genetic linkage analysis.

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[q] …….. is used to diagnose chromosomal imbalances (autosomal trisomies, sex chromosome disorders).

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[q] …….. is used for specific localization of genes and direct visualization of chromosomal anomalies at the molecular level:

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[f]IEZsdW9yZXNjZW5jZSBpbiBzaXR1IGh5YnJpZGl6YXRpb24u

[q] …… is an immunologic test used to detect the presence of either a specific antigen (HBsAg) or antibody (anti-HBs) in a patient’s blood sample. Direct tests for the antigen directly, while indirect tests for the antibody (thus indirectly testing for the antigen).

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[f]IEVuenltZS1saW5rZWQgaW1tdW5vc29yYmVudCBhc3NheS4=

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